Dr. Rubin is the principal of West Eastman High School in the second CGI/live-action film Alvin and the Chipmunks: The Squeakquel. She is an ecstatic Chipmunks fan and persuades them to perform in a music competition to save the school's music department. After she and the student body hear The Chipettes sing, she is pressured by them and Ian Hawke to allow a sing-off between the two groups. She becomes very emotional when The Chipmunks lose the chance to represent the school.

Alvin didn't believe she was a fan until she showed The Chipmunks a tattoo of them on her bicep. She states that she got it at their concert in Denver on her birthday.

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